Table of Contents
What is the role of Rhodamine 123?
Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. This product has been used to assay mitochondrial membrane potential in populations of apoptotic cells. For Research Use Only. Not for use in diagnostic procedures.
How does dihydrorhodamine test work?
The DHR (dihydrorhodamine) (flow cytometry test) and NBT (nitroblue tetrazolium) tests are used to diagnose CGD. Both work in a similar way. They check if someone’s blood cells are producing the enzyme NADPH oxidase, which plays a vital role in the way white blood cells fight infection.
What is DHR 123?
Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. After cell uptake, DHR 123 is oxidized by ROS into a fluorescent compound (Rhodamine 123).
How is granulomatous disease diagnosed?
Your doctor may order several tests to diagnose CGD , including: Neutrophil function tests. Your doctor may conduct a dihydrorhodamine 123 (DHR) test or other tests to see how well a type of white blood cell (neutrophil) in your blood is functioning. Doctors usually use this test to diagnose CGD .
What does neutrophil oxidative burst mean?
Neutrophil Oxidative Burst Test (or Chronic Granulomatous Disease (CGD) Test) is a measure of neutrophil oxidation is a useful assay in the diagnosis of chronic granulomatous disease and is also a useful means to determine the overall metabolic integrity of phagocytosing neutrophils.
What is DHR test?
The dihydrorhodamine, or DHR, test, is a blood test used to test for chronic granulomatous disease, CGD. It can also detect the type of CGD or carrier status. The DHR Collection Kit is used to collect and ship blood samples to be tested for CGD using the DHR test.
What does MitoTracker measure?
MitoTracker green (MTG) and nonylacridine orange (NAO) are fluorescent probes which have been commonly used to assess mitochondrial mass. This is based on the assumption that both MTG and NAO accumulate in mitochondria regardless of the mitochondrial transmembrane potential (ΔΨm).
What disease causes granulomas?
Chronic granulomatous disease (CGD) is a genetic disorder in which white blood cells called phagocytes are unable to kill certain types of bacteria and fungi. People with CGD are highly susceptible to frequent and sometimes life-threatening bacterial and fungal infections.
How do you measure oxidative burst?
The first method depends on the reduction of cytochrome c, which can be assessed by photometry, while the second method relies on changes in the fluorescence properties of dihydrorhodamine 123, which can be assessed by flow cytometry.
What cells do oxidative burst?
3.1. The oxidative burst (OB) or respiratory burst was first reported as the rapid release of reactive oxygen species (ROS) such as hydrogen peroxide and superoxide from animal immune cells, for example, neutrophils and monocytes, to degrade internalized pathogens.
What is CGD?
How does a MitoTracker work?
MitoTracker probes passively diffuse across the plasma membrane and then accumulate in active mitochondria in a potential-dependent manner. Subsequently, it covalently binds the thiol groups of the cysteine residues of mitochondrial proteins (Chazotte, 2011).
What does MitoTracker green stain?
MitoTracker Green (MTG) is a mitochondrial-selective fluorescent label commonly used in confocal microscopy and flow cytometry. It is expected that this dye selectively accumulates in the mitochondrial matrix where it covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues.