Table of Contents
What is ultraviolet light box used for in gel electrophoresis?
UV light boxes are used to help visualize results from of DNA and RNA analysis through gel electrophoresis. Molecules subjected to gel electrophoresis create a pattern of bands on a gel medium as they move. Scientists can interpret the pattern to obtain the results of the analysis.
Why is UV light used in DNA electrophoresis?
It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized.
What is blue light Transilluminator?
A blue light transilluminator is an instrument designed to observe and shoot a wide range of DNA samples. The device is highly sensitive and utilizes a harmless and bright blue LED light to view dyes, eliminating the need for ultraviolet (UV) transilluminators, which can be dangerous to skin and eyes.
How do you make DNA visible by UV light after gel electrophoresis?
To make the DNA visible in the gel, ethidium bromide is added to the gel solution and the buffer (it can also be left out of the gel and buffer; staining of the gel can be done in that case after the gel run..).
Does DNA fluoresce under UV light?
To actually see the DNA products, you MUST use some sort of stain or dye. DNA is colorless and is NOT visible under normal light (or UV light) without a dye or stain.
How does a UV Transilluminator work?
UV transilluminators work by emitting high levels of UV radiation through the viewing surface where the wet agarose gels are placed. Since the gels are previously stained with a fluorescent dye which binds to the nucleic acid, exposing them to a UV light source causes the dye to fluoresce.
What is gel documentation system?
A gel doc, also known as a gel documentation system, gel image system or gel imager, refers to equipment widely used in molecular biology laboratories for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels.
Which dye is used to Visualise DNA under UV light?
Ethidium bromide
Ethidium bromide is likely the most well-known dye used for visualizing DNA. It can be used in the gel mixture, the electrophoresis buffer, or to stain the gel after it is run. Molecules of the dye adhere to DNA strands and fluoresce under UV light, showing you exactly where the bands are within the gel.
What is an electrophoresis apparatus?
The gel electrophoresis apparatus consists of a gel, which is often made from agar or polyacrylamide, and an electrophoretic chamber (typically a hard plastic box or tank) with a cathode (negative terminal) at one end and an anode (positive terminal) at the opposite end.
What is gel electrophoresis machine?
Gel electrophoresis instruments are used to separate nucleic acids and proteins based on their size and charge. Used in forensic, molecular biology, genetics, and microbiology labs, gel electrophoresis instruments are used to run and compare DNA samples.
What color is UV light?
UV light has a shorter wavelength than visible light. Purple and violet light have shorter wavelengths than other colors of light, and ultraviolet has even shorter waves than violet does; so ultraviolet is sort of “purpler-than-purple” light or “beyond violet” light.
Why is SYBR Green used in gel electrophoresis?
SYBR Green I is used for staining dsDNA and ssDNA. SYBR Green II will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain.
What is the difference between SYBR Green and SYBR Safe?
Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye? All Invitrogen SYBR dyes have similar spectral properties, but have different chemical compositions. SYBR Safe DNA Gel Stain was specifically developed as a safer alternative to ethidium bromide.
What is vertical gel electrophoresis?
Vertical gel electrophoresis contains stacking gel and resolving gel. The stacking gel concentrates proteins that are loaded into the well so that the proteins can start to migrate at the same time. After stacking, the resolution gel separate proteins based on the molecular size.